TY - GEN UR - http://lib.ugent.be/catalog/pug01:975896 ID - pug01:975896 LA - eng TI - Evaluation of automated sample preparation, retention time locked GC-MS and automated data analysis for the metabolomic study of Arabidopsis species PY - 2010 PB - 2010 AU - Qun, Gu AU - David, Frank UGent 800000057672 AU - Jorge, Lucie AU - Lynen, Frederic WE07 801001133038 0000-0002-4690-7690 AU - Sandra, Koen AU - Rumpel, Klaus AU - Xu, Guowang AU - Sandra, Patrick AB - One of the most critical parameters in GC-MS application to metabolimic studies is the sample preparation. Often a combination of oximation and silylation is used and this method is applicable to acids (e.g. fatty acids), sterols, amines, amino acids, sugars, etc. Automation of the derivatisation process enhances repeatability and the time between sample preparation and analysis can be kept short and constant. For analysis, retention time locked (RTL) gas chromatography/mass spectrometry was used. For data analysis, AMDIS deconvolution followed by 2 multivariate statistical approaches were used and compared. Arabidopsis thaliana, a popular model organism in plant biology and genetics, was used for testing. The data analysis software allowed the detection of several features that were up- or downregulated in the different species. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 975896 | ||
| 005 | 20161219153536.0 | ||
| 008 | 100609s2010------------------------eng-- | ||
| 024 | a 1854/LU-975896 2 handle | ||
| 040 | a UGent | ||
| 245 | a Evaluation of automated sample preparation, retention time locked GC-MS and automated data analysis for the metabolomic study of Arabidopsis species | ||
| 260 | c 2010 | ||
| 520 | a One of the most critical parameters in GC-MS application to metabolimic studies is the sample preparation. Often a combination of oximation and silylation is used and this method is applicable to acids (e.g. fatty acids), sterols, amines, amino acids, sugars, etc. Automation of the derivatisation process enhances repeatability and the time between sample preparation and analysis can be kept short and constant. For analysis, retention time locked (RTL) gas chromatography/mass spectrometry was used. For data analysis, AMDIS deconvolution followed by 2 multivariate statistical approaches were used and compared. Arabidopsis thaliana, a popular model organism in plant biology and genetics, was used for testing. The data analysis software allowed the detection of several features that were up- or downregulated in the different species. | ||
| 598 | a C3 | ||
| 100 | a Qun, Gu u WE07 0 802000423907 9 FAC9FE52-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 700 | a David, Frank u UGent 0 800000057672 0 971496954621 9 F32E21F0-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 700 | a Jorge, Lucie | ||
| 700 | a Lynen, Frederic u WE07 0 801001133038 0 0000-0002-4690-7690 9 F52D4724-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 700 | a Sandra, Koen | ||
| 700 | a Rumpel, Klaus | ||
| 700 | a Xu, Guowang | ||
| 700 | a Sandra, Patrick u WE07 0 801000303888 9 F3A39052-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 650 | a Chemistry | ||
| 653 | a Fiehn library | ||
| 653 | a amino acid | ||
| 653 | a putrescine | ||
| 653 | a Retention time locking (RTL) | ||
| 653 | a arabidopsis thaliana leaf | ||
| 653 | a gas chromatography/mass spectrometry (GC/MS) | ||
| 653 | a metabolomics | ||
| 653 | a multivariate statistical analyses (MVA) | ||
| 773 | t 34th International symposium on Capillary Chromatography ; 7th GC×GC Symposium g Capillary Chromatography, 34th International symposium, Abstracts. 2010. p.175-175 q :<175 | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/975896/file/1009853 z [open] y poster-C24-Fiehnl_KS_adapt_Bess_checking.pdf | ||
| 920 | a confcontrib | ||
| Z30 | x WE 1 WE07 | ||
| 922 | a UGENT-WE | ||
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