TY - THES UR - http://lib.ugent.be/catalog/pug01:8531461 ID - pug01:8531461 LA - eng TI - Virus infection and host antiviral defense, a story between Israeli acute paralysis virus (IAPV) and bumblebees (Bombus terrestris) PY - 2017 SN - 9789463570206 PB - Ghent AU - Wang, Haidong UGent 000130340213 802001648329 AU - Smagghe, Guy promotor LA21 801000837388 0000-0001-8334-3313 AU - Meeus, Ivan copromotor LA21 001996034472 AB - The recent emerging of RNA viruses in bumblebees (Bombus spp.) caught scientific attention for its notorious reputation in another well-known pollinator species Apis mellifera. This calls for a comprehensive understanding of the interactions between these viruses and the bumblebee host. In this thesis, we investigated: 1) the symptom and tissue infection pattern of Israeli acute paralysis virus (IAPV) in bumblebee Bombus terrestris, 2) the involvement of the host innate immune system in the antiviral defense. 1) Systemic IAPV infection can be induced after both injecting and feeding of IAPV stock. During systemic infection, multiple B. terrestris tissues were infected and obvious paralysis symptoms (e.g. fore-legs paralysis) were observed. However, the occurrence of this paralysis symptoms cannot be explained by the virus tissue specific infections. 2) A single von Willebrand factor C-domain protein (SVC) was found to be involved in the antiviral defense of B. terrestris, which function is mediated by Dicer-2, a key component of the small interfering RNA (siRNA) pathway. Lower expression of antimicrobial peptides (AMP) genes were observed after SVC silencing, suggesting a potential links of SVC with AMPs. However, SVC does not seem to interact with AMPs induction upon IAPV infection. Besides, evidence of hemocytes necrosis but not apoptosis can be observed after IAPV infection in B. terrestris. Future studies towards understanding the role that cellular immunity plays in the host antiviral defense is needed. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 8531461 | ||
| 005 | 20180816080042.0 | ||
| 008 | 170918s2017------------------------eng-- | ||
| 020 | a 9789463570206 | ||
| 024 | a 1854/LU-8531461 2 handle | ||
| 040 | a UGent | ||
| 245 | a Virus infection and host antiviral defense, a story between Israeli acute paralysis virus (IAPV) and bumblebees (Bombus terrestris) | ||
| 260 | a Ghent, Belgium b Ghent University. Faculty of Bioscience Engineering c 2017 | ||
| 518 | a Public defense: 2017-09-18 16:00 | ||
| 520 | a The recent emerging of RNA viruses in bumblebees (Bombus spp.) caught scientific attention for its notorious reputation in another well-known pollinator species Apis mellifera. This calls for a comprehensive understanding of the interactions between these viruses and the bumblebee host. In this thesis, we investigated: 1) the symptom and tissue infection pattern of Israeli acute paralysis virus (IAPV) in bumblebee Bombus terrestris, 2) the involvement of the host innate immune system in the antiviral defense. 1) Systemic IAPV infection can be induced after both injecting and feeding of IAPV stock. During systemic infection, multiple B. terrestris tissues were infected and obvious paralysis symptoms (e.g. fore-legs paralysis) were observed. However, the occurrence of this paralysis symptoms cannot be explained by the virus tissue specific infections. 2) A single von Willebrand factor C-domain protein (SVC) was found to be involved in the antiviral defense of B. terrestris, which function is mediated by Dicer-2, a key component of the small interfering RNA (siRNA) pathway. Lower expression of antimicrobial peptides (AMP) genes were observed after SVC silencing, suggesting a potential links of SVC with AMPs. However, SVC does not seem to interact with AMPs induction upon IAPV infection. Besides, evidence of hemocytes necrosis but not apoptosis can be observed after IAPV infection in B. terrestris. Future studies towards understanding the role that cellular immunity plays in the host antiviral defense is needed. | ||
| 598 | a D1 | ||
| 100 | a Wang, Haidong u UGent 0 000130340213 0 802001648329 0 974191116584 | ||
| 700 | a Smagghe, Guy e promotor u LA21 0 801000837388 0 0000-0001-8334-3313 | ||
| 852 | x LA b LA21 | ||
| 700 | a Meeus, Ivan e copromotor u LA21 0 001996034472 0 801001471831 | ||
| 852 | x LA b LA21 | ||
| 650 | a Biology and Life Sciences | ||
| 653 | a bumblebee | ||
| 653 | a IAPV | ||
| 653 | a immunity | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/8531461/file/8531471 z [ugent] y Phd Thesis -Haidong Wang.pdf | ||
| 920 | a phd | ||
| 852 | x BW b LA03 | ||
| 922 | a UGENT-BW | ||
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