TY - JOUR UR - http://lib.ugent.be/catalog/pug01:5671942 ID - pug01:5671942 LA - eng TI - Enzyme-assisted extraction enhancing the phenolic release from cauliflower (Brassica oleracea L. var. botrytis) outer leaves PY - 2014 JO - (2014) JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY SN - 0021-8561 PB - 2014 AU - Huynh, Thai Nguyen 000111012961 802001450285 AU - Smagghe, Guy LA21 801000837388 0000-0001-8334-3313 AU - Gonzales, Gerard AU - Van Camp, John AU - Raes, Katleen LA23 801001275508 0000-0003-2382-120X AB - Phenolic compounds are highly present in byproducts from the cauliflower (Brassica oleracea L. var. botrytis) harvest and are thus a valuable source for valorization toward phenolic-rich extracts. In this study, we aimed to optimize and characterize the release of individual phenolic compounds from outer leaves of cauliflower, using two commercially available polysaccharide-degrading enzymes, Viscozyme L and Rapidase. As major results, the optimal conditions for the enzyme treatment were: enzyme/substrate ratio of 0.2% for Viscozyme L and 0.5% for Rapidase, temperature 35 degrees C, and pH 4.0. Using a UPLC-HD-TOF-MS setup, the main phenolic compounds in the extracts were identified as kaempferol glycosides and their combinations with different hydroxycinnamic acids. The most abundant components were kaempferol-3-feruloyldiglucoside and kaempferol-3-glucoside (respectively, 37.8 and 58.4 mg rutin equiv/100 g dry weight). Incubation of the cauliflower outer leaves with the enzyme mixtures resulted in a significantly higher extraction yield of kaempferol-glucosides as compared to the control treatment. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 5671942 | ||
| 005 | 20161219153857.0 | ||
| 008 | 140811s2014------------------------eng-- | ||
| 022 | a 0021-8561 | ||
| 024 | a 000339694300022 2 wos | ||
| 024 | a 1854/LU-5671942 2 handle | ||
| 024 | a 10.1021/jf502543c 2 doi | ||
| 040 | a UGent | ||
| 245 | a Enzyme-assisted extraction enhancing the phenolic release from cauliflower (Brassica oleracea L. var. botrytis) outer leaves | ||
| 260 | c 2014 | ||
| 520 | a Phenolic compounds are highly present in byproducts from the cauliflower (Brassica oleracea L. var. botrytis) harvest and are thus a valuable source for valorization toward phenolic-rich extracts. In this study, we aimed to optimize and characterize the release of individual phenolic compounds from outer leaves of cauliflower, using two commercially available polysaccharide-degrading enzymes, Viscozyme L and Rapidase. As major results, the optimal conditions for the enzyme treatment were: enzyme/substrate ratio of 0.2% for Viscozyme L and 0.5% for Rapidase, temperature 35 degrees C, and pH 4.0. Using a UPLC-HD-TOF-MS setup, the main phenolic compounds in the extracts were identified as kaempferol glycosides and their combinations with different hydroxycinnamic acids. The most abundant components were kaempferol-3-feruloyldiglucoside and kaempferol-3-glucoside (respectively, 37.8 and 58.4 mg rutin equiv/100 g dry weight). Incubation of the cauliflower outer leaves with the enzyme mixtures resulted in a significantly higher extraction yield of kaempferol-glucosides as compared to the control treatment. | ||
| 598 | a A1 | ||
| 100 | a Huynh, Thai Nguyen 0 000111012961 0 802001450285 0 977614416639 | ||
| 700 | a Smagghe, Guy u LA21 0 801000837388 0 0000-0001-8334-3313 | ||
| 700 | a Gonzales, Gerard u LA07 0 802001287813 | ||
| 700 | a Van Camp, John u LA23 0 801000900642 | ||
| 700 | a Raes, Katleen u LA23 0 801001275508 0 0000-0003-2382-120X | ||
| 650 | a Biology and Life Sciences | ||
| 653 | a Brassica oleracea | ||
| 653 | a release of phenolic compounds | ||
| 653 | a cauliflower outer leaves | ||
| 653 | a enzyme-assisted extraction | ||
| 653 | a kaempferol | ||
| 653 | a flavonoids | ||
| 653 | a BETA-GLUCOSIDASE | ||
| 653 | a BY-PRODUCTS | ||
| 653 | a FLAVONOIDS | ||
| 653 | a CELLULASE | ||
| 653 | a INHIBITION | ||
| 653 | a HYDROLYSIS | ||
| 653 | a POMACE | ||
| 653 | a WASTE | ||
| 653 | a TRANSGLYCOSYLATION | ||
| 653 | a GLUCOSINOLATE | ||
| 773 | t JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY g J. Agric. Food Chem. 2014. 62 (30) p.7468-7476 q 62:30<7468 | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/5671942/file/5671943 z [ugent] y Huynh2014-jafc.pdf | ||
| 920 | a article | ||
| 852 | x BW b LA16 | ||
| 922 | a UGENT-BW | ||
| 852 | x BW b LA03 | ||
| 922 | a UGENT-BW | ||
| 852 | x BW b LA07 | ||
| 922 | a UGENT-BW | ||
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