TY - JOUR UR - http://lib.ugent.be/catalog/pug01:4430428 ID - pug01:4430428 LA - eng TI - Role of the lower and upper intestine in the production and absorption of gut microbiota-derived PUFA metabolites PY - 2014 JO - (2014) PLOS ONE SN - 1932-6203 PB - 2014 AU - Druart, Céline AU - Neyrinck, Audrey M AU - Vlaeminck, Bruno WE11 001995057402 801001543468 0000-0002-6706-3693 AU - Fievez, Veerle LA22 801001124247 0000-0001-5042-6200 AU - Cani, Patrice D AU - Delzenne, Nathalie M AB - In vitro studies have suggested that isolated gut bacteria are able to metabolize PUFA into CLA (conjugated linoleic acids) and CLnA (conjugated linolenic acids). However, the bioavailability of fatty acid metabolites produced in vivo by the gut microbes remains to be studied. Therefore, we measured intestinal concentration and plasma accumulation of bacterial metabolites produced from dietary PUFA in mice, first injected with a lipoprotein lipase inhibitor, then force-fed with either sunflower oil (200 mu l) rich in n-6 PUFA or linseed oil (200 mu l) rich in n-3 PUFA. The greatest production of bacterial metabolites was observed in the caecum and colon, and at a much lesser extent in the jejunum and ileum. In the caecal content, CLA proportions were higher in sunflower oil force-fed mice whereas CLnA proportions were higher in linseed oil force-fed mice. The accumulation of the main metabolites (CLA cis-9, trans-11-18: 2 and CLnA cis-9, trans-11, cis-15-18:3) in the caecal tissue was not associated with their increase in the plasma, therefore suggesting that, if endogenously produced CLA and CLnA have any biological role in host metabolism regulation, their effect would be confined at the intestinal level, where the microbiota is abundant. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 4430428 | ||
| 005 | 20161221154136.0 | ||
| 008 | 140626s2014------------------------eng-- | ||
| 022 | a 1932-6203 | ||
| 024 | a 000330507300201 2 wos | ||
| 024 | a 1854/LU-4430428 2 handle | ||
| 024 | a 10.1371/journal.pone.0087560 2 doi | ||
| 040 | a UGent | ||
| 245 | a Role of the lower and upper intestine in the production and absorption of gut microbiota-derived PUFA metabolites | ||
| 260 | c 2014 | ||
| 520 | a In vitro studies have suggested that isolated gut bacteria are able to metabolize PUFA into CLA (conjugated linoleic acids) and CLnA (conjugated linolenic acids). However, the bioavailability of fatty acid metabolites produced in vivo by the gut microbes remains to be studied. Therefore, we measured intestinal concentration and plasma accumulation of bacterial metabolites produced from dietary PUFA in mice, first injected with a lipoprotein lipase inhibitor, then force-fed with either sunflower oil (200 mu l) rich in n-6 PUFA or linseed oil (200 mu l) rich in n-3 PUFA. The greatest production of bacterial metabolites was observed in the caecum and colon, and at a much lesser extent in the jejunum and ileum. In the caecal content, CLA proportions were higher in sunflower oil force-fed mice whereas CLnA proportions were higher in linseed oil force-fed mice. The accumulation of the main metabolites (CLA cis-9, trans-11-18: 2 and CLnA cis-9, trans-11, cis-15-18:3) in the caecal tissue was not associated with their increase in the plasma, therefore suggesting that, if endogenously produced CLA and CLnA have any biological role in host metabolism regulation, their effect would be confined at the intestinal level, where the microbiota is abundant. | ||
| 598 | a A1 | ||
| 100 | a Druart, Céline | ||
| 700 | a Neyrinck, Audrey M | ||
| 700 | a Vlaeminck, Bruno u WE11 0 001995057402 0 801001543468 0 0000-0002-6706-3693 | ||
| 700 | a Fievez, Veerle u LA22 0 801001124247 0 0000-0001-5042-6200 | ||
| 700 | a Cani, Patrice D | ||
| 700 | a Delzenne, Nathalie M | ||
| 650 | a Biology and Life Sciences | ||
| 653 | a BIOSYNTHESIS | ||
| 653 | a BACTERIA | ||
| 653 | a SECRETION | ||
| 653 | a OBESITY | ||
| 653 | a ISOMERS | ||
| 653 | a DIETARY-FAT | ||
| 653 | a HUMAN HEALTH | ||
| 653 | a MILK-FAT | ||
| 653 | a FATTY-ACIDS | ||
| 653 | a CONJUGATED LINOLEIC-ACID | ||
| 773 | t PLOS ONE g PLoS One. 2014. 9 (1) q 9:1< | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/4430428/file/4430436 z [open] y 785_-_PloOne9_2014_e87560.pdf | ||
| 920 | a article | ||
| 852 | x BW b LA13 | ||
| 922 | a UGENT-BW | ||
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