TY - CHAP UR - http://lib.ugent.be/catalog/pug01:4315404 ID - pug01:4315404 LA - eng TI - Cofactor profiling of the glucocorticoid receptor from a cellular environment PY - 2014 SN - 9781493913466 SN - 9781493913459 SN - 1064-3745 PB - New York AU - Desmet, Sofie GE07 000070218094 AU - Dejager, Lien 002001291872 801002052013 AU - Clarisse, Dorien GE01 GE07 002005212187 802001355006 0000-0002-2580-2291 AU - Thommis, Jonathan AU - Melchers, Diana AU - Bastiaensen, Niek AU - Ruijtenbeek, Rob AU - Beck, Ilse 002002753138 801001639357 AU - Libert, Claude WE14 801000697750 0000-0001-6408-036X AU - Houtman, Renée AU - Meijer, Onno C AU - De Bosscher, Karolien AU - Castoria, Gabriella editor AU - Auricchio, Ferdinando editor AB - The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor-coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol and data analysis. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 4315404 | ||
| 005 | 20180813143152.0 | ||
| 008 | 140228s2014------------------------eng-- | ||
| 020 | a 9781493913466 | ||
| 020 | a 9781493913459 | ||
| 022 | a 1064-3745 | ||
| 024 | a 1854/LU-4315404 2 handle | ||
| 024 | a 10.1007/978-1-4939-1346-6_8 2 doi | ||
| 040 | a UGent | ||
| 245 | a Cofactor profiling of the glucocorticoid receptor from a cellular environment | ||
| 260 | a New York, NY, USA b Springer c 2014 | ||
| 520 | a The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor-coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol and data analysis. | ||
| 598 | a B2 | ||
| 100 | a Desmet, Sofie u GE07 0 000070218094 0 802001319741 | ||
| 700 | a Dejager, Lien 0 002001291872 0 801002052013 0 975212730688 | ||
| 700 | a Clarisse, Dorien u GE01 u GE07 0 002005212187 0 802001355006 0 0000-0002-2580-2291 | ||
| 700 | a Thommis, Jonathan u GE07 0 802000923657 | ||
| 700 | a Melchers, Diana | ||
| 700 | a Bastiaensen, Niek | ||
| 700 | a Ruijtenbeek, Rob | ||
| 700 | a Beck, Ilse 0 002002753138 0 801001639357 0 971100483083 | ||
| 700 | a Libert, Claude u WE14 0 801000697750 0 0000-0001-6408-036X | ||
| 700 | a Houtman, Renée | ||
| 700 | a Meijer, Onno C | ||
| 700 | a De Bosscher, Karolien u GE07 0 801001033109 | ||
| 700 | a Castoria, Gabriella e editor | ||
| 700 | a Auricchio, Ferdinando e editor | ||
| 650 | a Medicine and Health Sciences | ||
| 653 | a MARCoNI. | ||
| 653 | a glucocorticoid receptor | ||
| 653 | a profiling | ||
| 653 | a corepressor | ||
| 653 | a coactivator | ||
| 653 | a coregulator | ||
| 653 | a nuclear receptors | ||
| 653 | a peptide microarray | ||
| 773 | t Steroid receptors : methods and protocols g Steroid receptors : methods and protocols. 2014. Springer. 1204 p.83-94 q 1204:<83 | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/4315404/file/5801770 z [ugent] y Desmet_et_al._MMB2014.pdf | ||
| 920 | a chapter | ||
| 852 | x GE b GE07 | ||
| 922 | a UGENT-GE | ||
| 852 | x VI b VIB | ||
| 922 | a UGENT-EA | ||
| 852 | x WE b WE14 | ||
| 922 | a UGENT-WE | ||
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