TY - JOUR UR - http://lib.ugent.be/catalog/pug01:214318 ID - pug01:214318 LA - eng TI - Epigenetic switch from posttranscriptional to transcriptional silencing is correlated with promoter hypermethylation PY - 2003 JO - (2003) PLANT PHYSIOLOGY SN - 0032-0889 PB - 2003 AU - Fojtova, Miloslava AU - Van Houdt, Helena AU - Depicker, Anna WE09 801000377650 0000-0003-0105-7407 AU - Kovarik, Ales AB - Changes in the distribution of methylcytosine residues along a transgene locus of tobacco (Nicotiana tabacum) in relation to the type of gene silencing were studied in parental plant leaves, calli, and regenerated plants derived thereof. Parental-silenced HeLo1 (hemizygous for locus 1) plants show posttranscriptional silencing of the residing nptII (neomycin phosphotransferase II) transgene and cytosine methylation restricted to the 3' end and center part of the transcribed region. Here, we report that with an increasing number of cell cycles, DNA methylation changes gradually, and methylation is introduced into the promoter during cell culture and more slowly in vegetatively propagated plants. After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical (CG and CNG) sites was almost complete within the 5' end of the nptII-transcribed region and the 35S promoter. Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3' end of the transcribed region when compared with locus 1. The newly established epigenetic patterns were stably transmitted from calli into regenerated plants and their progeny. The protein and steady-state RNA levels remained low in locus 1E, whereas with nuclear run-on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the methylated promoter became inactivated. The results suggest that a switch between posttranscriptional and transcriptional gene silencing could be a mechanism leading to irrevocable shut down of gene expression within a finite number of generations. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 214318 | ||
| 005 | 20161219154007.0 | ||
| 008 | 040504s2003------------------------eng-- | ||
| 022 | a 0032-0889 | ||
| 024 | a 000186644600029 2 wos | ||
| 024 | a 1854/LU-214318 2 handle | ||
| 024 | a 10.1104/pp.103.023796 2 doi | ||
| 040 | a UGent | ||
| 245 | a Epigenetic switch from posttranscriptional to transcriptional silencing is correlated with promoter hypermethylation | ||
| 260 | c 2003 | ||
| 520 | a Changes in the distribution of methylcytosine residues along a transgene locus of tobacco (Nicotiana tabacum) in relation to the type of gene silencing were studied in parental plant leaves, calli, and regenerated plants derived thereof. Parental-silenced HeLo1 (hemizygous for locus 1) plants show posttranscriptional silencing of the residing nptII (neomycin phosphotransferase II) transgene and cytosine methylation restricted to the 3' end and center part of the transcribed region. Here, we report that with an increasing number of cell cycles, DNA methylation changes gradually, and methylation is introduced into the promoter during cell culture and more slowly in vegetatively propagated plants. After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical (CG and CNG) sites was almost complete within the 5' end of the nptII-transcribed region and the 35S promoter. Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3' end of the transcribed region when compared with locus 1. The newly established epigenetic patterns were stably transmitted from calli into regenerated plants and their progeny. The protein and steady-state RNA levels remained low in locus 1E, whereas with nuclear run-on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the methylated promoter became inactivated. The results suggest that a switch between posttranscriptional and transcriptional gene silencing could be a mechanism leading to irrevocable shut down of gene expression within a finite number of generations. | ||
| 598 | a A1 | ||
| 100 | a Fojtova, Miloslava | ||
| 700 | a Van Houdt, Helena u WE09 0 801000903571 | ||
| 700 | a Depicker, Anna u WE09 0 801000377650 0 0000-0003-0105-7407 | ||
| 700 | a Kovarik, Ales | ||
| 650 | a Biology and Life Sciences | ||
| 653 | a REPEATS | ||
| 653 | a RNA | ||
| 653 | a TRANSGENE | ||
| 653 | a ARABIDOPSIS | ||
| 653 | a PLANTS | ||
| 653 | a GENE | ||
| 653 | a PETUNIA-HYBRIDA | ||
| 653 | a CYTOSINE METHYLATION | ||
| 653 | a DIRECTED DNA METHYLATION | ||
| 653 | a DE-NOVO METHYLATION | ||
| 773 | t PLANT PHYSIOLOGY g Plant Physiol. 2003. 133 (3) p.1240-1250 q 133:3<1240 | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/214318/file/4144682 z [ugent] y 214318_Fojtova_et_al.__2003_PlantPhysiol133_1240.pdf | ||
| 920 | a article | ||
| 852 | x VI b VIB | ||
| 922 | a UGENT-EA | ||
| 852 | x WE b WE09 | ||
| 922 | a UGENT-WE | ||
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