TY - JOUR UR - http://lib.ugent.be/catalog/pug01:2134046 ID - pug01:2134046 LA - eng TI - Nuclear inclusion of nontargeted and chromatin-targeted polystyrene beads and plasmid DNA containing nanoparticles PY - 2011 JO - (2011) MOLECULAR PHARMACEUTICS SN - 1543-8384 PB - 2011 AU - Symens, Nathalie UGent 002002043624 AU - Walczak, Rudolf AU - Demeester, Jo FW01 801000332584 0000-0003-2436-1524 AU - Mattaj, Iain AU - De Smedt, Stefaan CA05 FW01 801000783939 0000-0002-8653-2598 AU - Remaut, Katrien FW01 001996458040 AB - The nuclear membrane is one of the major cellular barriers in the delivery of plasmid DNA (pDNA). Cell division has a positive influence on the expression efficiency since, at the end of mitosis, pDNA or pDNA containing complexes near the chromatin are probably included by a random process in the nuclei of the daughter cells. However, very little is known about the nuclear inclusion of nanoparticles during cell division. Using the Xenopus nuclear envelope reassembly (XNER) assay, we found that the nuclear enclosure of nanoparticles was dependent on size (with 100 and 200 nm particles being better included than the 500 nm ones) and charge (with positively charged particles being better included than negatively charged cr polyethyleneglycolated (PEGylated) ones) of the beads. Also, coupling chromatin-targeting peptides to the polystyrene beads or pDNA complexes improved their inclusion by 2- to 3-fold. Upon microinjection in living HeLa cells, however, nanoparticles were never observed in the nuclei of cells postdivision but accumulated in a specific perinuclear region, which was identified as the lysosomal compartment. This indicates that nanoparticles can end up in the lysosomes even when they were not delivered through endocytosis. To elucidate if the chromatin binding peptides also have potential in living cells, this additional barrier first has to be tackled, since it prevents free particles from being present near the chromatin at the moment of cell division. ER -Download RIS file
| 00000nam^a2200301^i^4500 | |||
| 001 | 2134046 | ||
| 005 | 20181113145430.0 | ||
| 008 | 120606s2011------------------------eng-- | ||
| 022 | a 1543-8384 | ||
| 024 | a 000295347500033 2 wos | ||
| 024 | a 1854/LU-2134046 2 handle | ||
| 024 | a 10.1021/mp200120v 2 doi | ||
| 040 | a UGent | ||
| 245 | a Nuclear inclusion of nontargeted and chromatin-targeted polystyrene beads and plasmid DNA containing nanoparticles | ||
| 260 | c 2011 | ||
| 520 | a The nuclear membrane is one of the major cellular barriers in the delivery of plasmid DNA (pDNA). Cell division has a positive influence on the expression efficiency since, at the end of mitosis, pDNA or pDNA containing complexes near the chromatin are probably included by a random process in the nuclei of the daughter cells. However, very little is known about the nuclear inclusion of nanoparticles during cell division. Using the Xenopus nuclear envelope reassembly (XNER) assay, we found that the nuclear enclosure of nanoparticles was dependent on size (with 100 and 200 nm particles being better included than the 500 nm ones) and charge (with positively charged particles being better included than negatively charged cr polyethyleneglycolated (PEGylated) ones) of the beads. Also, coupling chromatin-targeting peptides to the polystyrene beads or pDNA complexes improved their inclusion by 2- to 3-fold. Upon microinjection in living HeLa cells, however, nanoparticles were never observed in the nuclei of cells postdivision but accumulated in a specific perinuclear region, which was identified as the lysosomal compartment. This indicates that nanoparticles can end up in the lysosomes even when they were not delivered through endocytosis. To elucidate if the chromatin binding peptides also have potential in living cells, this additional barrier first has to be tackled, since it prevents free particles from being present near the chromatin at the moment of cell division. | ||
| 598 | a A1 | ||
| 700 | a Symens, Nathalie u UGent 0 002002043624 0 802000213335 9 F9EE74C2-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 700 | a Walczak, Rudolf | ||
| 700 | a Demeester, Jo u FW01 0 801000332584 0 0000-0003-2436-1524 9 F3B8D52A-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 700 | a Mattaj, Iain | ||
| 700 | a De Smedt, Stefaan u CA05 u FW01 0 801000783939 0 0000-0002-8653-2598 9 F4836E84-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 700 | a Remaut, Katrien u FW01 0 001996458040 0 801001559737 9 F98B2FB6-F0ED-11E1-A9DE-61C894A0A6B4 | ||
| 650 | a Medicine and Health Sciences | ||
| 653 | a GOLD NANOPARTICLES | ||
| 653 | a MITOTIC-ACTIVITY | ||
| 653 | a AIRWAY EPITHELIAL-CELLS | ||
| 653 | a MEDIATED GENE-TRANSFER | ||
| 653 | a nuclear envelope reassembly | ||
| 653 | a mitosis | ||
| 653 | a nuclear exclusion | ||
| 653 | a pDNA delivery | ||
| 653 | a HeLa cells | ||
| 653 | a Xenopus laevis | ||
| 653 | a cell division | ||
| 653 | a nuclear enclosure | ||
| 653 | a IN-VITRO | ||
| 653 | a EXPRESSION | ||
| 653 | a CYCLE | ||
| 653 | a TRANSFECTION | ||
| 653 | a EFFICIENCY | ||
| 653 | a CELLULAR UPTAKE | ||
| 773 | t MOLECULAR PHARMACEUTICS g Mol. Pharm. 2011. 8 (5) p.1757-1766 q 8:5<1757 | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/2134046/file/2134047 z [ugent] y SymensN_Nuclear_inclusion_Mol_Pharm_2011.pdf | ||
| 856 | 3 Full Text u https://biblio.ugent.be/publication/2134046/file/3127561 z [open] y Nuclear.pdf | ||
| 920 | a article | ||
| Z30 | x FW 1 FW01 | ||
| 922 | a UGENT-FW | ||
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